Martine De Herdt

78 Chapter 3 Supplementary figure S3: Immunoreactivities of the five tested C-terminal MET antibodies under FFPE conditions in respect to MET mRNA expression levels of the antibody validation cell line panel. A. representative immunoreactivities of all antibodies with all cell lines and the corresponding negative controls (NCs). B. membranous (M), cytoplasmic (C), and nuclear (N) immunohistochemical reactivity. C. qRT-PCR results showing average MET fluorescence standardized to average HPRT1 fluorescence. D. Legend for observed mRNA expression levels and immunohistochemical reactivities. It should be noted that pictures used to present the immunoreactivities obtained with D1C2 and CVD13 are the same as those presented in Figure 1D.

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