175 MET, ECD shedding, and loss of E-cadherin Supplementary figures Supplementary figure 1: Photographs representing the staining patterns – uniform negative, gradient towards the periphery, uniform positive and gradient towards the center – observed for A2H2-3* (A, 20X objective) and NCH-38** (B, 20X objective) using the two-dimensional scoring system. * For A2H2-3 the gradient towards center staining pattern was not observed (indicated by a grey square). ** For NCH-38 the gradient towards periphery staining pattern was not observed (indicated by a grey square). Supplementary figure 2: Photographs representative of D1C2 immunoreactivity across one cancer, illustrating the heterogeneity of staining patterns across WTSs. A. Cancer area completely representative for the uniform positive staining pattern (20X objective). B. Cancer area representative for a 50:50 mixture of the uniform positive and gradient towards periphery staining pattern (20X objective). C. Cancer area completely representative for the gradient towards periphery staining pattern (20X objective). 5
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