147 MET, ECD shedding, and loss of E-cadherin periphery staining pattern tends to cover larger areas of the cancer (Supplementary figures 3I through L). Comparison of staining patterns observed with D1C2 and NCH-38 Although pairwise comparisons of the distributions and medians of the scores of inverse staining patterns of D1C2 and NCH-38 show that there are significant differences for D1C2 uniform negative versus NCH-38 uniform positive and D1C2 gradient toward the periphery versus NCH-38 gradient toward the center staining patterns, the comparisons also show that there is no significant difference between the distributions and – identical – medians (10.0%) of the D1C2 uniform positive and NCH-38 uniform negative staining pattern (Supplementary figures 3A through D and I through L, Supplementary table 18, Figures 2A through C). These results indicate that D1C2 uniform positive patches of tumor are likely to be subjective to down-regulation of E-cadherin. Comparison of staining patterns observed with D1C2 and A2H2-3 Pairwise comparison, of the distributions and medians of the corresponding scores per respective staining pattern for D1C2 and A2H2-3, shows that they differ significantly for the three staining patterns (Supplementary figures 3A through C and 3E through G, Supplementary table 19). The median for uniform negativity observed using A2H2-3 (65.0%) is significantly higher than that observed using D1C2 (15.0%). The opposite is true for the uniform positive (5.0% versus 10.0%) and gradient toward the periphery staining patterns (20.0% versus 40.0%, respectively, Supplementary figures 3D and H, Figures 2D through F). These results suggest that ECD shedding occurs in both the uniform positive and gradient toward the periphery fraction, resulting in overall higher uniform negativity of A2H2-3 (Supplementary figure 4). 5
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