Anna Brouwer

Abstract Purpose This study sought to investigate whether there is an optimal position of the Dawson, Trick, and Litzkow (DTL) electrodes when measuring the full-field electroretinogram (ERG) for monitoring purposes. Methods In 200 uveitis patients an extended light adapted (LA) ERG protocol was measured twice, incorporating the International Society for Clinical Electrophysiology of Vision standards. First a LA ERG was measured with the DTL in the lower lid position (LLP), and thereafter in the fornix position. Differences in amplitudes and implicit times of a-waves, b-waves and the 30 Hz peak were investigated. Intraclass correlation coefficients (ICC) as well as coefficients of variation (CoV) were calculated, to assess both reliability and relative variability between the two DTL positions. Results Implicit times showed no statistically significant differences between the two DTL positions. As expected, amplitudes at the different stimulus strengths were 1.12 - 1.19 higher in the LLP, but there were no significant differences in the CoV between the two DTL positions. The ICC was high for the b-wave and 30 Hz flicker response (0.842 - 0.979), but lower for the a-wave, especially for amplitudes (0.584 - 0.716). Conclusions For monitoring purposes in patients, we conclude that based on relative variability, no position is preferable above the other. However, because in most diseases amplitudes are decreased, the LLP may be chosen because it yields higher amplitudes. Whatever the choice, it is important to ensure that the DTL position remains stable during an ERG recording. 140 Chapter 7