Chapter 4 —— 89 —— and X.W.). Research in the author’s laboratory is supported by the Prinses Beatrix Spierfonds, the Dutch Duchenne Parent Project, the Horizon Europe Programme, and the Dutch Research Council (NWO)—Open Technology Programme. Conflict of interest statement: None declared. References 1. Pacesa, M.; Pelea, O.; Jinek, M.. Past, present, and future of CRISPR genome editing technologies. Cell 2024, 187, 1076–1100. 2. Chen, X.; Gonçalves, M.A.F.V.. DNA, RNA, and protein tools for editing the genetic information in Human cells. iScience 2018, 6, 247–263. 3. Liao, H.; Wu, J.; VanDusen, N.J.; Li,Y.; Zheng, Y.. CRISPR/Cas9-mediated homologydirected repair for precise gene editing. Mol. Ther. Nucleic Acids 2024, 35, 102344. 4. He, X.; Tan, C.; Wang, F.; Wang, Y.; Zhou, R.; Cui, D.; You, W.; Zhao, H.; Ren, J.; Feng, B.. Knock-in of large reporter genes in human cells via CRISPR/Cas9-induced homology-dependent and independent DNA repair. Nucleic Acids Res. 2016, 44, e85. 5. Suzuki, K.; Tsunekawa, Y.; Hernandez-Benitez, R.; Wu, J.; Zhu, J.; Kim, E.J.; Hatanaka, F.; Yamamoto, M.; Araoka,T.; Li,Z.; et al. In vivo genome editing via CRISPR/Cas9 mediated homology-independent targeted integration. Nature 2016, 540, 144–149. 6. Nakade, S.; Tsubota, T.; Sakane, Y.; Kume, S.; Sakamoto, N.; Obara, M.; Daimon, T.; Sezutsu, H.; Yamamoto, T.; Sakuma, T.; et al. Microhomology-mediated end-joiningdependent integration of donor DNA in cells and animals using TALENs and CRISPR/Cas9. Nat. Commun. 2014, 5, 5560. 7. Chen, X.; Janssen, J.M.; Liu, J.; Maggio, I.; Jong, A.E.J.; Mikkers, H.M.M.; Gonçalves, M.. In trans paired nicking triggers seamless genome editing without double-stranded DNA cutting. Nat. Commun. 2017, 8, 657. 8. Yao, X.; Wang, X.; Hu, X.; Liu, Z.; Liu, J.; Zhou, H.; Shen, X.; Wei, Y.; Huang, Z.; Ying,W.; et al. Homology-mediated end joining-based targeted integration using CRISPR/Cas9. Cell Res. 2017, 27, 801–814. 9. Zhang, J.P.; Li, X.L.; Li, G.H.; Chen, W.; Arakaki, C.; Botimer, G.D.; Baylink, D.; Zhang, L.; Wen, W.; Fu, Y.W.; et al. Efficient precise knockin with a double cut HDR donor after CRISPR/Cas9-mediated double-stranded DNA cleavage. Genome Biol. 2017, 18, 35. 10. Chen, X.; Gonçalves, M.A.. Engineered viruses as genome editing devices. Mol. Ther. 2016, 24, 447–457.
RkJQdWJsaXNoZXIy MTk4NDMw