Chapter 4 —— 64 —— Figure 6. Investigating the role of AAV donor structures on the specificity and fidelity of genome editing. (A) Experimental setup. Homology arms in regular and sc AAV donors are drawn in relation to the AAVS1 acceptor sequence before and after gene targeting. PCR amplicons diagnostic for HDR-mediated formation of exogenousendogenous chromosomal junctions from the telomere and centromere side are also
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