Chapter 5 —— 138 —— Supplementary Table S12. Primers and PCR mixtures used for junction PCR analysis of ATP1A1::mScarlet (Figures 5F) Target Primer code Primers (5’ → 3’) / final concentrations (µM) dNTP (mM) MgCl2 (mM) GoTaq Flexi Buffer GoTaq (Units) Amplicon size (bp) mScarlet #2120 ACGGTGTAGTCCTCGTTGTG / 0.4 0.4 1 1x 1.25 1095 #1648 TCTCGCACATTCTTCACGTC / 0.4 jT.ATP1A1 #2229 ACTACAGGGCGTGCATACAG / 0.4 0.4 1 1x 1.25 1199 #2230 CCCACAACGAGGACTACACC / 0.4 jC.ATP1A1 #2234 GGTGACCTACCAGCCAAACT / 0.4 0.4 1 1x 1.25 945 #2235 CTTGGAAAAGGCGCAACCC / 0.4 Supplementary Table S13. PCR cycling parameters used for junction PCR analysis of ATP1A1::mScarlet (Figures 5F) Target Initial denaturation Denaturation Annealing Elongation Cycles Final elongation mScarlet 95 ℃ 95 ℃ 63 ℃ 72 ℃ 35 72 ℃ 5 min 30 sec 30 sec 1 min 5 min jT.ATP1A1 95 ℃ 95 ℃ 65 ℃ 72 ℃ 35 72 ℃ 5 min 30 sec 30 sec 1 min 1 min 30 sec jC.ATP1A1 95 ℃ 95 ℃ 65 ℃ 72 ℃ 35 72 ℃ 5 min 30 sec 30 sec 1 min 1 min 30 sec
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