Chapter 5 —— 136 —— Supplementary Table S8. Primers and PCR mixtures used for genotyping of LMNA and ATP1A1 (Figures 3C, 3E) Target Primer code Primers (5’ → 3’) / final concentrations (µM) dNTP (mM) MgCl2 (mM) GoTaq Flexi Buffer GoTaq (Units) Amplicon size (bp) mScarlet #2328 CACGAGTTCGAGATCGAGGG / 0.4 0.2 1 1x 1.25 573 #2329 TTCGTACTGTTCCACCACGG / 0.4 jT.LMNA #2332 GAACAGTACGAACGCTCCGA / 0.4 0.2 1 1x 1.25 781 #2333 CTGGGTGCCCAGAGTTCTTC / 0.4 jC.LMNA #2330 TGAGTCACACTGATGGGCAC / 0.4 0.2 1 1x 1.25 1263 #2331 GGTGTAGTCCTCGTTGTGGG / 0.4 ATP1A1 #2225 CCCCTCCCGACAAAATCAATAC / 0.4 0.2 1 1x 1.25 1275 #2228 TAGCACCACACCCAGGTACA / 0.4 Supplementary Table S9. PCR cycling parameters used genotyping of LMNA and ATP1A1 (Figures 3C, 3E) Target Initial denaturation Denaturation Annealing Elongation Cycles Final elongation mScarlet 95 ℃ 95 ℃ 62.9 ℃ 72 ℃ 30 72 ℃ 5 min 30 sec 30 sec 30 sec 5 min jT.LMNA 95 ℃ 95 ℃ 62.9 ℃ 72 ℃ 30 72 ℃ 5 min 30 sec 30 sec 40 sec 5 min jC.LMNA 95 ℃ 95 ℃ 62.9 ℃ 72 ℃ 30 72 ℃ 5 min 30 sec 30 sec 1 min 5 min ATP1A1 95 ℃ 95 ℃ 64 ℃ 72 ℃ 30 72 ℃ 5 min 30 sec 30 sec 1 min 5 min
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