Chapter 5 —— 135 —— Supplementary Table S6. Primers and PCR mixtures used for genotyping of AAVS1 and ATP1A1 (Figures 2D) Target Primer code Primers (5’ → 3’) / final concentrations (µM) dNTP (mM) MgCl2 (mM) GoTaq Flexi Buffer GoTaq (Units) DMSO Amplicon size (bp) EGFP #978 GAGCTGGACGGCGA CGTAAACG / 0,4 0.4 1 1× 1.25 596 #979 CGCTTCTCGTTGGG GTCTTTGCT / 0,4 jT.AAVS1 #986 AACCCCAACCCCGT GGAAG / 0,4 0.4 1 1× 1.25 2% 1666 #1004 GCACCGTCCGCTTC GAG / 0,4 jC.AAVS1 #1046 CGACAACCACTACC TGAGCA / 0,4 0.4 1 1× 1.25 1712 #1047 GACCTGCCTGGAGA AGGAT / 0,4 ATP1A1 #2225 CCCCTCCCGACAAA ATCAATAC / 0.4 0.4 1 1× 1.25 1275 #2228 TAGCACCACACCCA GGTACA / 0.4 Supplementary Table S7. PCR cycling parameters used for genotyping of AAVS1 and ATP1A1 (Figures 2D) Target Initial denaturation Denaturation Annealing Elongation Cycles Final elongation EGFP 95 ℃ 95 ℃ 72 ℃ 35 72 ℃ 5 min 30 sec 20 sec 5 min jT.AAVS1 95 ℃ 95 ℃ 61 ℃ 72 ℃ 35 72 ℃ 5 min 30 sec 30 sec 1 min 30 sec 1 min 30 sec jC.AAVS1 95 ℃ 95 ℃ 67 ℃ 72 ℃ 35 72 ℃ 5 min 30 sec 30 sec 1 min 30 sec 1 min 30 sec ATP1A1 95 ℃ 95 ℃ 64 ℃ 72 ℃ 40 72 ℃ 5 min 30 sec 30 sec 1 min 20 sec 5 min
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