Chapter 5 —— 133 —— Supplementary Tables Supplementary Table S1. Titres of AAV vectors generated for this study. Plasmid code AAV/AdVP vector Titre BI17 AAV-HRS1.A1 1.86E+08 TU/mL BI19 AAV-HRLMN.A1 1.14E+11 GC/mL BI38 AAV-HRA1.IN17 8.61E+07 TU/mL U67 AdVP.C9KARA 5.43E+07 GC/mL Supplementary Table S2. Primers and PCR mixtures used for clonal screening of AAVS1 (Figures 1E, 1F and Supplementary figure S2) Target Primer code Primers (5’ → 3’) / final concentrations (µM) 2X Phire Tissue Direct PCR Master mix µl DMSO Amplicon size (bp) GFP #978 GAGCTGGACGGCGACGTAAACG / 0,5 10 596 #979 CGCTTCTCGTTGGGGTCTTTGCT / 0,5 jT.AAVS1 #986 AACCCCAACCCCGTGGAAG / 0,5 10 2% 1666 #1004 GCACCGTCCGCTTCGAG / 0,5 jC.AAVS1 #1046 CGACAACCACTACCTGAGCA / 0,5 10 1712 #1047 GACCTGCCTGGAGAAGGAT / 0,5 Supplementary Table S3. PCR cycling parameters used for clonal screening of AAVS1 (Figures 1E, 1F and Supplementary figure S2) Target Initial denaturation Denaturation Annealing Elongation Cycles Final elongation GFP 98 ℃ 98 ℃ 72 ℃ 35 72 ℃ 5 min 5 sec 20 sec 2 min jT.AAVS1 98 ℃ 98 ℃ 72 ℃ 35 72 ℃ 5 min 7 sec 30 sec 2 min jC.AAVS1 98 ℃ 98 ℃ 72 ℃ 72 ℃ TD 0,5 ℃ decrease/cycle*10 72 ℃ 5 min 7 sec 7 sec 32 sec 98 ℃ 67 ℃ 72 ℃ 25 2 min 7 sec 7 sec 32 sec
RkJQdWJsaXNoZXIy MTk4NDMw