Chapter 5 —— 130 —— Supplementary Figure S2. Characterization of AAVS1 and ATP1A1 co-targeting resulting from selectable AAV donor delivery. PCR genotyping of individual genetically-modified cell clones generated via AAV-HRS1.A1 transduction of HeLa cells transfected with a Cas9-encoding plasmid. Prior to clone isolation, the HeLa cell populations were expanded either in the presence or absence of ouabain. Nuclease-free water (W) and DNA from unmodified HeLa cells (-) provided for negative controls; genomic DNA from sorted EGFP-positive HeLa cells exposed to Cas9 and AAV-HRS1.A1, served as positive control (+). The AAVS1 and ATP1A1 genotype screens involved junction
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