Chapter 3 │ Page 90 Figure 8: Assessment of secreted DAMPs after NTP-CDDP treatment. Stimulation of ATP secretion 4 hours and 24 hours after treatment was moderate, while strong increases in HMGB1 release could be noted 2 and 3 days post therapy. Bar plots show DAMP levels in the supernatant normalized to untreated controls. Data presented as mean ± SEM (n = 3, 3 independent repeats with pooled replicates). Statistical significance for all pairwise comparisons was determined via linear mixed model analysis with Tukey’s correction for multiple testing. (* p ≤ 0.05; *** p ≤ 0.001; **** p ≤ 0.0001). Hence, these findings advocate for the beneficial interaction between NTP and CDDP in promoting ICD, not only via early- and mid-stage presented DAMPs, but also by persisting in the later stages, thereby enforcing immunogenicity throughout the entire process of this regulated cell death (Figure 9).
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