Chapter 3 │ Page 85 Figure 4: HNSCC-PDO tumor kinetics after NTP or CDDP monotherapy. HNSCC_001T and HNSCC_007T were exposed to di erent treatment regimens and monitored for 72 hours with live-cell imaging. Images were analyzed using the in-house developed Orbits® platform. Data presented as mean ± SEM (n = 15, 3 independent repeats). Statistical significance for pairwise comparison to untreated controls was determined via linear mixed model analysis with Dunnett’s correction for multiple testing. (* p ≤ 0.05; ** p ≤ 0.01; *** p ≤ 0.001; **** p ≤ 0.0001). organoid HNSCC_007T displayed a higher sensitivity towards NTP and CDDP than HNSCC_001T. While NTP had limited e ects on the tumor kinetics from HNSCC_001T and only high doses of CDDP could reach statistical significance (≥9.28µM, p < 0.0001), HNSCC_007T responded to the lowest doses of CDDP tested (0.5µM, p ≤ 0,0197) and was also more susceptible to NTP application (UT vs 1000 Hz, p = 0.0562). Next, we tested the combination of NTP with two CDDP concentrations that had no considerable impact as standalone therapy, and followed them up for 96 hours to be able to capture the full development of the combinatorial reaction (Figure 5). We
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