Chapter 2 │ Page 57 Appendix APPENDIX 2.A Cell Survival analysis 24 hours post NTP exposure after incubation at di erent temperatures. To perform ambient light-exposure experiments, e ects of changes in incubation temperature needed to be taken into account. Overall, cell survival after NTP treatment was not or minimally a ected by this environmental change, for both parental as transduced SC263 cells. Statistical significance was determined with a Two-Way ANOVA and a post hoc Tukey’s multiple comparison test. * p ≤ 0.05. APPENDIX 2.B Baseline intracellular ROS level comparison between low- and high-passaged cellular counterparts. Quantification of the mean green fluorescence intensity of the images (GCU) and calculation of fold changes between young and older counterparts showed that continuous culturing had no e ect on basal ROS levels in the parental cell lines. In contrast, a significant elevation of baseline ROS levels in the transduced cell line following prolonged passaging (SC263T-PH) was observed, compared to their younger counterpart SC263T-PL. Data are represented as mean±SEM of 3 independent repeats (n=10-15). Statistical significance was determined using a One-Way ANOVA with a Dunn's multiple comparisons test. *** p ≤ 0.001. BC FG I CM FF FC IO R FFO S U F IRXC I C I R ns C C C C
RkJQdWJsaXNoZXIy MTk4NDMw