Chapter 2 │ Page 48 Figure 3: Cancer cell survival analysis 24 hours after di erent NTP treatment energies. (a) At low passages, no significant di erences in NTP sensitivity were reported between the transduced (SC263T) and parental cells (SC263), while after higher passages (b), transduced SC263T-PH cells showed significantly reduced survival following NTP treatment at 0.4 J and higher, compared to the non-transduced counterparts. (c) The passaging of parental SC263 cells did not a ect their sensitivity to NTP, but in contrast, (d) NTP sensitivity increased in the transduced cells, with significantly lower survival rates in the SC263T-PH compared to SC263T-PL at 0.9 J of NTP treatment energy and higher. Data are presented as mean±SEM of 3 independent repeats (n=15-20). Statistical significance was determined with a Two-Way ANOVA and a post hoc Tukey’s multiple comparison test. * p ≤ 0.05, *** p ≤ 0.001, **** p ≤ 0.0001. 3.2 Phototoxicity from Di erent Light Sources A ects NTP Treatment Response and Cellular Physiology To investigate which types of light excitation were able to induce the observed phototoxicity and increased NTP sensitivity, both parental and fluorescentlytransduced cell lines were exposed to ambient light before NTP treatment, and analyzed for cell survival, 24 hours post NTP exposure. The e ects of temperature changes (37°C vs room temperature, RT) were taken into account and did not a ect
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