Chapter 1 │ Page 22 Figure 2: Schematic overview of the thesis outline. This figure shows the contribution and structure of the di erent aspects discussed throughout the PhD dissertation. In chapter 2 (research article), the impact of confounding ROS production in fluorescence-based live cell imaging for experimental read-outs was determined, as it is crucial to ensure accurate experimental design before evaluation of redoxbased therapies like NTP. I found out that stable incorporation of a fluorescent reporter can increase intracellular ROS levels, leading to aberrant cellular behavior and di erences in therapeutic response in the context of NTP exposure. We incorporated this knowledge, and adapted our experimental models according to this for the next chapter, chapter 3 (research article), in which I evaluated the immunogenetic benefit of NTP addition to standard-of-care HNSCC therapy. Using
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