Chapter 4 │ Page 149 Supplementary Figure 3: NTP application results in minor changes in CD47 expression in several HNSCC cell lines. Quantification of the amount of CD47 expression after exposure to di erent regimes of NTP. Results are depicted as mean fluorescence intensity minus FMO control, normalized to untreated controls (normalized (Norm) ΔMFI), immediate (0h) and one day (24h) post-treatment for all 3 HNSCC cell lines. Data are represented as mean ± SEM, with individual values shown (n = 5). Statistical significance between untreated cells and the treated conditions was determined using the generalized linear mixed model with post hoc Dunnett’s test (* p ≤ 0.05). Outliers were calculated with the Grubbs’ Test. C D D C EF I MN N TU N N N B C C B
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