Chapter 4 │ Page 147 Supplementary Figures Supplementary Figure 1: Flow cytometry plots of untreated Cal27 HNSCC cells, representing the gating strategy of all treatment conditions and cell lines throughout the experiment. HNSCC cell lines (Cal27, SCC61, and SCC22B) were stained for the target (anti-HLA-C, anti-HLA-E, anti-CD155, anti-CD122, anti-CD73, or anti-MICA/B) in the PE channel, and counterstained with a live-dead stain (LIVE/DEAD™ Fixable Near-IR, APCCy7). A single cell population was obtained by doublet removal in the forward scatter height and area (FSC-H vs FSC-A) plot, whereafter singlets were gated on morphology in the forward and side scatter area (FSC-A vs SCC-A) plot. Expression of the target-PE was determined in the APC-Cy7- live cell population.
RkJQdWJsaXNoZXIy MTk4NDMw