Chapter 4 │ Page 138 Figure 7: NTP reduces the expression of key targets in the TIGIT axis and CD73 immediately after treatment exposure. Flow cytometric quantification of the TIGIT receptor ligands (a) CD155 and (b) CD112, and (c) immune checkpoint CD73. Expression levels are depicted as mean fluorescence intensity minus FMO control, normalized to untreated controls (Norm ΔMFI), immediate (0h) and one day (24h) post-treatment for all three HNSCC cell lines. Data are represented as mean ± SEM, with individual values shown (n = 5). Statistical significance between untreated cells and the treated conditions was determined using the generalized linear mixed model with post hoc Dunnett’s test (* p ≤ 0.05; ** p ≤ 0.01; *** p ≤ 0.001). Outliers were calculated with the Grubbs’ Test.
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