Chapter 4 │ Page 136 Besides immediate oxidative e ects at the cell surface, NTP exerts more pleiotropic and downstream e ects on cancer cells [18, 31, 59, 60]. Therefore, the expression levels of these MHC-I molecules were also measured 24h after NTP application. This time point was selected to clearly identify the immediate chemical (e.g., oxidative) of plasma treatment and later biological e ects at 24 hours. Indeed, including an intermediate time point (e.g., 4 or 12 hours) would complicate this distinction, as it would likely reflect a mixture of diminishing chemical, but also early biological responses, making it challenging to delineate the independent contributions of each. Cellular responses in HLA-C and HLA-E expression toward NTP were limited for all cell lines, except for a significant upregulation in HLA-E expression (3.07; p = 0.0455) at a low NTP regimen in the Cal27 cell line (Figure 6b, left panel).
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