Chapter 4 │ Page 125 Figure 2: Amino acid structures. Chemical structures of sulfur-containing (top) and aromatic (bottom) amino acids with their oxidized forms (i.e. structures with atoms in red). 2.4. Cell Culture Three human head and neck squamous cell carcinoma cell lines, Cal27, SCC61 and SCC22B, were used in this study. The Cal27 cell line was obtained from American Type Culture Collection (ATCC, Rockville, MD, USA), the SCC61 cell line was kindly provided by Prof. Dr. Sandra Nuyts (University Hospital Leuven, Leuven, Belgium), and SCC22B cells were kindly provided by Prof. Dr. Olivier De Wever (Laboratory of Experimental Cancer Research, Ghent University Hospital, Ghent, Belgium). Cell lines were commercially available and no ethical approval was required. Cells were cultured in Dulbecco's Modified Eagle Medium (DMEM, Life Technologies), supplemented with 10% fetal bovine serum (FBS, Life Technologies), 1% L-glutamine (Life Technologies), and 1% Penicillin/Streptomycin (P/S; Life Technologies). Cell cultures were incubated in a humidified atmosphere at 37°C and 5% CO2 conditions, and passaged when 80% confluence was reached to maintain exponential growth. Cell identity was confirmed via short tandem repeat profiling, and regular checks for mycoplasma infection were performed.
RkJQdWJsaXNoZXIy MTk4NDMw