Chapter 3 98 Mono-culture analysis is performed by first averaging the observations for each timepoint (per line), followed by an overall average for DIV21 and DIV26. Differences in mean levels were analysed using one-way ANOVA, which was deemed appropriate after confirming normal residuals (Shapiro-Wilk test) and homogeneity of variances (Brown-Forsythe test). To ensure transparency, individual observations for each cell line are plotted in Supplementary Fig. 2, allowing visual assessment of data distribution and variability across groups. All test results for mono-culture are detailed in Supplementary Table III. All statistical testing was conducted in GraphPad Prism (v.10.2.0). FUNDING This work was supported by the European Joint Programme on Rare Diseases (EJPRD19201, NG4LEUKO). COMPETING INTERESTS The authors report no competing interests. AUTHOR CONTRIBUTIONS LMLK, EM and CR generated cortical neurons. LMLK performed neuronal maturation and sample collection for sequencing. SB, EV and JR performed neuronal maturation and subsequent neurite outgrowth and microtubule dynamics analysis. BC analysed RNA sequencing experiments. VMH, AG and MS supervised the project. LMLK visualized the data and wrote the chapter in consultation with other authors.
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