Cortical interneuron development is affected in 4H leukodystrophy 75 2 SUPPLEMENTARY FIGURES Supplementary Figure 1. Effect of activity modulators on neuronal cultures. Graphs show that the number of spikes in control and 4H neurons significantly decreased after treatment with TTX (A), APV (B) and DNQX (C). Control cells DNQX before 398.1 ± 111.5 after 221.3 ± 103.0, Z = -2.201, P = 0.028; control cells APV before 174.6 ± 121.7 after 107.7 ± 109.2, t(5) = 5.369, P = 0.003; 4H cells DNQX before 834.3 ± 424.1, after 126.6 ± 65.1, t(9) = 3.412, P = 0.008, 4H cells APV before 174.6 ± 121.7, after 107.7 ± 109.2, Z = -2.803, P = 0.005. Addition of solvents to cultures did not influence network activity in any of the conditions (D-J). (G-I) GABA ant. = GABA antagonists bicuculline and gabazine. * = P < 0.05, ** = P < 0.01. Data points and lines represent data per well, with lines with the same color representing wells from the same individual.
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