Chapter 2 50 Figure 5. 4H neurons show high network activity and a reduced response to GABA antagonists. (A) Brightfield image of neurons plated on MEA. (B) Schematic example of spike and burst detection from MEA recording. (C) Representative example of baseline activity at 14 weeks post plating over 60 seconds on different electrodes in control and 4H neuronal cultures. Of the 4H neurons 3 out of 4 show an increased spike frequency (D), burst count (E) and network burst count (F) than control lines, although the differences are not statistically significant. (G-J) After addition of GABA antagonists bicuculline and gabazine, control neurons show a significantly increase in spikes (H), burst (I) and network bursts (J), while 4H neurons only show an increase in spikes (I). (K-L) Addition of GABA decreased the number of spikes in both control and 4H neurons. Scale bar = 200 μm. * = P < 0.05, ** = P < 0.01 (D-F) Data-points and lines represent averaged data per patient, with the light areas representing SD per patient. (H, I, J, L) data points and lines represent data per well, with lines with the same colour representing wells from the same individual.
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