Chapter 2 48 Figure 4. Cortical neurons of 4H patients show a decreased generation of GABAergic synapses. (A) Representative images of immunostainings on control and 4H neurons. Neurons are stained for dendrites (MAP2, left, blue), all synapses (Synaptophysin1, middle left, green) and GABAergic synapses (VGAT, middle right, red). Dendritic (B) and axonal (C) density were not changed in 4H neurons compared to controls. The number of synapses per dendritic length was normal in 4H (D), while the percentage of GABAergic synapses was significantly decreased in 4H neurons (E). Scalebar = 25 μm , px = pixel, * = P < 0.05. (B-E) Open circles represent average per individual patient/control, filled circles represent the mean per genotype. Error bars represent SEM.
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