Chapter 5 180 Supplementary Figure 2: Independent experiments show that hPSC-derived cells are microglia-like A-D) Microglia-like cells from different hPSC lines are all Iba1+ at week 10 of experiment. Scale bars 50 μm. E) In mono-culture all microglia-like cells from line GFP+ H09 (green) went on to express TMEM119 (red). Nuclei are stained using DAPI (blue). Scale bars 100 μm. F) Microglia-like cells in mono-culture also went on to express microglia markers Iba1, P2RY12, and TMEM119 on RNA level. Supplementary Figure 3: Representative images used for high-content analysis Scale bars A&B 50 μm, C&D 100 μm.
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