Chapter 5 162 Fig. 2. Microglia significantly affect morphological features of human cortical neurons in vitro. Illustrative image of nuclear debris as stained by DAPI (black) in co-cultures A) without or B) with microglia. Morphological features nuclear debris (C), mature NeuN positive neurons (D), Neurofilament+ axonal (E) and MAP2+ dendritic segments (F) were significantly affected (p < 0.0001, Fisher’s method). A table containing further post-hoc testing results shows several axonal (G) and dendritic (H) morphology parameters, that were normalized to the amount of neurons, to be significantly different between cultures with and without microglia. I) Graph displaying reduced density of synaptophysin positive puncta. For each timepoint N = at least 8 observations on 4 neuronal backgrounds. For each measurement WPP = weeks post plating (of neurons). Error bars represent the standard error of the mean. Scale bar 100 µm. *P < 0.05,**P < 0.01, ***P < 0.001.
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