Chapter 5 160 Figure 1: Long survival of human PSC-derived microglia-neuron co-cultures. A) Schematic representation of the experimental set-up. Stars indicating timepoints of cellomics immunofluorescent investigation. Representative immunofluorescent images of nuclei (DAPI, blue) neurons (MAP2, white) and microglia (GFP, green) at 12 weeks after plating of neurons H01 (B), hvs088 (C), hvs420 (D) and hvs451a (E). Quantification of microglia number (F), roundness (G), size (H), length (I), width (J) and ratio of width to length (K). Circles representing means for each neuron type for the three timepoints together. The observations for each timepoint are also represented by rectangle for timepoint 1, diamond for timepoint 2 and triangle for timepoint 3. For each timepoint N = 24 observations on 4 different neuronal backgrounds. WPP = weeks post plating (of neurons). Error bars represent the standard error of the mean. Scale bars 100 µm.
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