Towards a 3D spheroid system for modelling leukodystrophies 151 4 SUPPLEMENTARY TABLES Supplementary table I: iPSC lines. Line ID Donor Sex, Age Cell Origin hPSCreg ID Disease status Genotype Isogenic Pair Repr. method Origin Institute (City, Country) C1 HD1.2 F, adult Fibroblasts, Life tech., HDF C0135C TIGETi001A Healthy - mRNA IRCCs San Raffaele Scientific Institute (MLN, IT) C2 HD2.2 M, neonatal Fibroblasts, Life tech., HDF C0045C Healthy - A mRNA IRCCs San Raffaele Scientific Institute (MLN, IT) C3 hvs420 M, 21y Fibroblasts, Amsterdam UMC VUi036-A Healthy - Lentiviral Vrije Universiteit (AMS, NL) C4 C107 M, 34y Healthy - Institute of Reconstructive Neurobiology (BNN, DE) C5 C16 M, 79y Healthy - Institute of Reconstructive Neurobiology (BNN, DE) P1 KI H4 M, neonatal iPSC HD2.2 GLD GALC, c.1657G>A / c.1657G>A A mRNA IRCCs San Raffaele Scientific Institute (MLN, IT) P2 KO X19 M, neonatal iPSC HD2.2 GLD GALC KO A mRNA IRCCs San Raffaele Scientific Institute (MLN, IT) P3 GLD5.2 F, 1y Fibroblasts, Gaslini Biobank FFF0042010 GLD GALC, c.1657G>A / c.1657G>A mRNA IRCCs San Raffaele Scientific Institute (MLN, IT) P4 hvs449a M, 24y Patient-derived fibroblasts, Amsterdam UMC VUi029-A 4H POLR3A c.1771-6C>G & c.3205C>T (p.Arg1069Trp) Sendai Vrije Universiteit (AMS, NL) P5 hvs450a F, 2y Patient-derived fibroblasts, Amsterdam UMC VUi030-A 4H POLR3A c.1771-7C>G & c.1048+5G>T Sendai Vrije Universiteit (AMS, NL) P6 KO E27 M, 34y CD ASPA Institute of Reconstructive Neurobiology (BNN, DE) P7 KO E30 M, 34y CD ASPA Institute of Reconstructive Neurobiology (BNN, DE) P8 KO E70 M, 34y CD ASPA Institute of Reconstructive Neurobiology (BNN, DE) 4H: 4H Leukodystrophy, GLD: Globoid Leukodystrophy, CD: Canavan Disease. MLN: Milan, IT: Italy, AMS: Amsterdam, NL: The Netherlands, BNN: Bonn, DE: Germany. Life tech.: Life Technologies. Underlined isogenic pairs are the origin lines.
RkJQdWJsaXNoZXIy MTk4NDMw