Chapter 4 118 Figure 3: Addressing variance between spheroids using single cell mRNA sequencing data. A) PC plot showing the variance explained by PC1 and PC2. Underlined and italic line names form isogenic lines. Experimental batches are indicated with batch 1: none additions, batch 2: ‘, batch 3: “, batch 4: ‘“, batch 5: .., batch 6:. B-F) Cell clusters with significant differences in percentage of cells per cluster between d100 and d150. G) UMAP showing differences in cellular composition based on disease status of spheroids. H-J) Cell clusters with significant differences in percentage of cells per cluster between healthy donor (HD) and leukodystrophy spheroids (either GLD, 4H, or CD). *P < 0.05,**P < 0.01, ***P < 0.001.
RkJQdWJsaXNoZXIy MTk4NDMw