Chapter 4 114 Figure 1: Characterization of OL spheroids and their cell composition. A) Schematic representation of experimental set-up. B) Spheroid diameter per cell line, shaded ribbons represent the average standard deviation of the lines. Representative immunofluorescent (IF) image and quantification of the positive area for C) neuron marker (Neurofilament, NF in red), D) inhibitory neuron marker (GABA in white), E-G) oligodendrocyte precursor (Olig2 in white) and myelinating oligodendrocytes (MBP in green). H) Electron microscopy image of compacted myelin around an axon. I) Representative IF image showing GFAP expression, with quantification of GFAP+ area between HD and LD spheroids over time. *P < 0.05,**P < 0.01, ***P < 0.001, **** P < 0.0001.
RkJQdWJsaXNoZXIy MTk4NDMw