Investigating neuron intrinsic defects in 4H and Globoid Leukodystrophy 103 3 ◀ Supplementary Figure 2: Neuronal Features in Mono-cultures by line. Quantification of A) Neurite length, B) Axon length, C) EB3 comet density, D) EB3 Comet Velocity, E) Mitochondrial motility, F) Mitochondrial motility, G) Distance travelled by mitochondria, H) Speed of moving mitochondria. Scatter plots represent individual observations, with horizontal lines showing the mean for each cell line. Gray dots for observations on DIV 2 or 3 and black dots for DIV 7 or 8. Bar graphs in G and H figures summarize the group mean for either Control, 4H or Globoid Leukodystrophy with individual dots for cell line means. F) Direction of mitochondrial movement are depicted by percentage of mitochondria moving in both (filled bars), retrograde (diagonal striped bars) or anterograde (clear bars) direction. Error bars represent the standard deviation. Supplementary Figure 3: Principal Component Analysis (PCA) on bulk mRNA-sequencing of monoculture neurons. A) PCA of all samples generated from all genes, B) PCA from all genes without KO X19 sample and C) PCA of control and 4H samples only. GLD samples in purple, 4H in blue and control in black. Triangles represent male donors and circles female donors. Italicized labels indicate isogenic lines. Underlined line names are used to distinguish which lines originate from the Amsterdam institute.
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