Investigating neuron intrinsic defects in 4H and Globoid Leukodystrophy 101 3 SUPPLEMENTARY FIGURES Supplementary Figure 1: Quantification of GalCer, LysoGlcCer, and GlcCer levels in control (Ctrl) and GLD cells. Levels of GalCer (A), LysoGlcCer (B), and GlcCer (C) in iPSCs, DIV0 neurons, rat astrocytes and in DIV21 co-cultures. Each dot represents an individual sample, with control (black) and GLD (purple) groups shown. Rat astrocyte samples are included as additional reference for co-cultures. And comparison of GalCer (D), LysoGlcCer (E), and GlcCer (F) levels in iPSCs and DIV7 neurons in mono-culture from control (black) and GLD (purple) groups. Horizontal lines indicate group means. Lipid quantifications are presented in pmol/gram. Note the log scale for all panels, reflecting the wide range of detected concentrations.
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