199 Diagnostic utility of molecular and imaging biomarkers 2 malignant histology, ni FNAC sample ICC stain and assessment method malignant PTC FVPTC FTC FTC-OV MTC other n.s. FFPE cell blocks CK-19: positive if >50% of cells stained (strong stain) 44% 8 two destained cytology smears Ki-67: positive if ≥10% of cells stained (high proliferative index). Laminin: visual, positive if strong stain. 32% 8 one smear in cytolyt and centrifuged on slide, one unstained air-dried smear Gal-3: positive if >20% cells with staining of cytoplasm and/or plasma membrane (moderate to strong stain) 35% 9 3 FFPE cell blocks from FNAC needle washings Gal-3: visual, jointly by two reviewers, positive if staining of cytoplasm, membrane or nucleus 60% 3 thin-layer cytology smears Gal-3 / HBME-1 / RET: positive if ≥50% cells with cytoplasmic staining 46% 16 1 FFPE cell blocks Gal-3 / HBME-1 / CK19 / KS positive if ≥10% of cells stained (moderate to strong stain); TPO positive if <50% of cells stained. 60% 39 19 14 3 FFPE cell blocks CD57 (Leu7) / GLUT-1: positive if >10% cells stained for both markers (moderate to strong stain) 50% 3 4 cell blocks Gal-3: positive if >10% cells with cytoplasmic stain 4% 1 1 cytology smears Gal-3: visual, by two blinded independent pathologists, positive if any single epithelial cell with cytoplasmic or nucleio-cytoplasmic staining 35% 1 1 4 FFPE cell blocks Gal-3: positive if >5% cells with eminent cytoplasmic staining (focal to diffuse stain) 35% 86 15 22 40 FFPE cell blocks Gal-3: positive if any epithelial cell with cytoplasmic staining (weakly positive to positive) 15% 7 2 6 FFPE cell blocks cycline D1 and D3: (1) predefined positivity if ≥25% cells stained, versus (2) ROC-curve analysis to determine ideal cutoff 37% 4 15 FFPE cell blocks Gal-3 / HBME-1 / emerin: positive stain n.o.s. 53% 1 16 9 2 FNAC specimens n.o.s. Gal-3: positive stain n.o.s. 48% 4 12 5 1 7 liquid based cytology slides HBME-1 / CK19: positive if >70% cells stained for both markers 34% 16 liquid based cytology slides Gal-3 / HBME-1: positive if ≥50% cells with cytoplasmic staining 22% 10 1 liquid based cytology slides CK-19 (ni=31) / HBME-1 (ni=27): positive if ≥30% cells stained. Ki-67 (ni=34): positive if ≥1% cells stained. ROC curve analysis to determine ideal cutoff 16% 1 3 2 liquid based cytology slides HBME-1 / TROP-2: positive if >70% cells stained 18% 9 3 liquid based cytology slides (n=22) and cytology smears (n=29) Positive triple stain, with positive gal-3 if >30% / HBME-1 if >10% / p27 if <25% cells stained 44% 12 6 4 34.9% 89 172 69 60 0 53 16
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