145 Diagnostic utility of molecular and imaging biomarkers 2 other inclusion and exclusion criteria FNAC Sample RAS molecular technique TN; histological f/u available additional preoperative US-FNAC n.o.s. HRAS, KRAS, NRAS 2x PCR master mix TN; FNAC sample with adequate nucleic acids FNAC sample n.o.s. KRAS, NRAS PCR and direct sequencing TN; FNAC with sufficient epithelial cells and nucleic acids for molecular analysis; histological f/u available residual material from FNAC and needle washout HRAS, KRAS, NRAS miRinform® Thyroid Test suspicious TN >5mm, FNAC sample positive for thyroglobulin FNAC needle washout HRAS, KRAS, NRAS HRM analysis and sequencing TN; no Hürthle cell cytology; histological f/u available FNAC needle washout HRAS, KRAS, NRAS miRInform® Thyroid Test adult with TN ≥1cm; FNAC with sufficient nucleic acids for molecular analysis; correct specimen handling and shipping; no duplicate or contaminated specimen additional FNAC aspirate HRAS, KRAS, NRAS miRinform® Thyroid Test TN; BRAF mutation negative FNAC smears HRAS, KRAS, NRAS real-time PCR with primers for BRAF, direct sequencing for RAS if BRAF was negative TN; air-dried FNAC slide available; histological f/u and FFPE samples available for mutation analysis air-dried FNAC smears HRAS, KRAS, NRAS HRM analysis for BRAF, KRAS and NRAS, pyrosequencing for BRAF and HRAS/KRAS/NRAS TN n.o.s. FNAC smears HRAS, KRAS, NRAS PCR and pyrosequencing TN; sufficient DNA quality and quantity FNAC needle washout KRAS, NRAS Pyrosequencing TN; sufficient nucleic acids for molecular analysis; histological f/u available residual material from two FNAC passes HRAS, KRAS, NRAS ThyroSeq® v2 TN; size >1cm; histological f/u available air-dried FNAC smears HRAS, KRAS, NRAS Real-time PCR, HRM and pyrosequencing adults; TN; negative history of radiation exposure nor family history of thyroid carcinoma; histological f/u available FNAC cell block HRAS, KRAS, NRAS PCR and pyrosequencing adult with solitary cold TN ≥10mm; suspicious on US; normal TSH and calcitonin; histologic f/u available representative archived FNAC slide HRAS, KRAS, NRAS Direct sequencing TN; histological f/u available if BRAF or RAS mutation detected or size >2cm or suspicious US features or resection upon patient request. FNAC slides HRAS, KRAS, NRAS PCR and pyrosequencing TN; sufficient DNA for molecular analysis; histological f/u available cell blocks, or FNAC smears if DNA from cell block <10ng HRAS, KRAS, NRAS Commercially available 50-gene panel (AmpliSeq™ Cancer Hotspot Panel v2) TN n.o.s. residual material from 1 or 2 FNAC passes HRAS, KRAS, NRAS ThyroSeq® v2.1 TN; suspicious US features; histological or cytological f/u available additional needle pass HRAS, KRAS, NRAS PCR and direct sequencing for BRAF, real-time PCR and HRM for RAS TN n.o.s. n.s. HRAS, KRAS, NRAS miRinform® Thyroid Test
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