257 Synthesis and discussion resolved five “Ca. Methanoperedens” MAGs and one putative syntrophic SRB belonging to the class Desulfobacterota QYQD01. Moreover, we detect distinct MHCs and/or OmcZ-like nanowires that could be driving this interaction. We encourage to employ bioreactors to focus on S-AOM incubations with inocula from Lake Cadagno sediments. We suggest that combining in situ sediment metatransriptomics of sediments with marked methane oxidation rates could help define which are the most active EET or Direct interspecies electron transfer (DIET) mechanisms employed by “Ca. Methanoperedens” and SRB to sustain an interaction between them. Metatranscriptomics could also allow to investigate if Group III Dsr-LP might play a role in sulfite detoxification in Lake Cadagno’s “Ca. Methanoperedens” under syntrophic growth conditions. Additionally, exploring the mechanisms of sialic acid in forming tight conductive aggregates with SRB partners could also be valuable. Complementary investigations on the mineralogy of the sediment could provide better insights on the potential electron acceptors that are available in the sediment. 8
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