Maider Junkal Echeveste Medrano

228 Chapter 7 from Lake Cadagno. Metatranscriptomic and metaproteomic studies could be conducted on the sediment to determine which “Ca. Methanoperedens” and SRB species are most active, identify the preferred MHC proteins, and investigate whether Group III Dsr-LP plays a role in sulfide-derived sulfite detoxification. Furthermore, analyzing the mineralogy of Lake Cadagno could provide insights into other available metal electron acceptors for “Ca. Methanoperedens” spp. in situ. Data availability Raw sequence data for 16S rRNA amplicon sequencing are made available at NCBI under the BioProject ID PRJNA497531 with the accession numbers SRR15689035SRR15689041. Raw metagenomic data and MAGs recovered from the sediment and manganese oxide incubation co-assemblies have been uploaded to the European Nucleotide Archive with project number PRJEB81702. Authors contribution Conceptualization, MJEM (equal), GS (equal), JZ (equal); Data Curation, MJEM (equal), GS (equal), PL (supporting); Formal Analysis, MJEM (equal), GS (equal), LAB (supporting); Funding Acquisition, JZ (equal), CUW (equal), ISA (equal), MSMJ (equal), GS (supporting); Investigation, MJEM (equal), GS (equal), JZ (equal); Methodology, MJEM (equal), GS (equal), JZ (equal), PL (supporting); Resources, MJEM (equal), GS (equal), JZ (equal), PL (supporting); Supervision, JZ (equal), CUW (equal), ISA (equal), MSMJ (equal); Visualization, MJEM (lead), GS (supporting); Writing - Original Draft Preparation, MJEM (lead), JZ (supporting); Writing – Review & Editing, MJEM (equal), GS (equal), JZ (equal), CUW (equal), PL (supporting), LAB (supporting), ISA (supporting), MSMJ (supporting). Acknowledgements We thank Andy Leu for the bioinformatic data support and discussion. We acknowledge Thomas Kuhn for carbon isotopic analysis.

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