222 Chapter 7 c DTXE01 UWMA-0184 [GCA 012962595.1] c QYQD01 SpSt-1152 [GCA 011046395.1] Sed MAG c QYQD01 c QYQD01 DeMMO3_14 [GCA 020053325.1] c QYQD01 PowLak16_MAG11 [GCA 007280345.1] c JACQYL01|g JACQYL01 [GCA.016208165.1] c Syntrophia|o Syntrophales c Desulfomonilia c Thermodesulfobacteria|o Thermodesulfobacteriales c Dissulfuribacteria|o Dissulfuribacterales c Desulfobulbia|o Desulfobulbales c Desulfovibrionia|o Desulfovibrionales c Desulfofervidia|o Desulfofervidales c Desulfobacteria c DSM-4660|o Desulfatiglandales c JADFXK01|g JADFXK01 c RBG-13-43-22|o RBG-13-43-22|f RBG-13-43-22 c Syntrophobacteria c Desulfarculia c Desulfobaccia|o Desulfobaccales c Zymogenia|o Zymogenales 100 100 100 98 100 100 92 100 100 100 100 100 100 99 100 100 96 100 0.1 10% 90% 55% 45% 62/1170 Methanoperedenaceae SRA out of 62 62/113 Desulfobacterota c. QYQDO1 SRA Methanoperedenaceae & Desulfobacterota c. QYQDO1 Co-existence not detected B Iron-rich subsurface Cadagno Sediment Hydrothermal vent SOURCE Co-existence: Methanoperedenaceae CYTOSOLIC HYDROGENASE NiFe Group 3c Present Absent A Meromictic lake water Hydrothermal sediment 1 2 3 1 2 3 c DTXE01 UWMA-0184 [GCA 012962595.1] c QYQD01 SpSt-1152 [GCA 011046395.1] Sed MAG c QYQD01 c QYQD01 DeMMO3_14 [GCA 020053325.1] c QYQD01 PowLak16_MAG11 [GCA 007280345.1] c JACQYL01|g JACQYL01 [GCA.016208165.1] c Syntrophia|o Syntrophales c Desulfomonilia c Thermodesulfobacteria|o Thermodesulfobacteriales c Dissulfuribacteria|o Dissulfuribacterales c Desulfobulbia|o Desulfobulbales c Desulfovibrionia|o Desulfovibrionales c Desulfofervidia|o Desulfofervidales c Desulfobacteria c DSM-4660|o Desulfatiglandales c JADFXK01|g JADFXK01 c RBG-13-43-22|o RBG-13-43-22|f RBG-13-43-22 c Syntrophobacteria c Desulfarculia c Desulfobaccia|o Desulfobaccales c Zymogenia|o Zymogenales 100 100 100 98 100 100 92 100 100 100 100 100 100 99 100 100 96 100 0.1 10% 90% 55% 45% 62/1170 Methanoperedenaceae SRA out of 62 62/113 Desulfobacterota c. QYQDO1 SRA Methanoperedenaceae & Desulfobacterota c. QYQDO1 Co-existence not detected B Iron-rich subsurface Cadagno Sediment Hydrothermal vent SOURCE Co-existence: Methanoperedenaceae CYTOSOLIC HYDROGENASE NiFe Group 3c Present Absent A Meromictic lake water Hydrothermal sediment 1 2 3 1 2 3 c DTXE01 UWMA-0184 [GCA 012962595.1] c QYQD01 SpSt-1152 [GCA 011046395.1] Sed MAG c QYQD01 c QYQD01 DeMMO3_14 [GCA 020053325.1] c QYQD01 PowLak16_MAG11 [GCA 007280345.1] c JACQYL01|g JACQYL01 [GCA.016208165.1] c Syntrophia|o Syntrophales c Desulfomonilia c Thermodesulfobacteria|o Thermodesulfobacteriales c Dissulfuribacteria|o Dissulfuribacterales c Desulfobulbia|o Desulfobulbales c Desulfovibrionia|o Desulfovibrionales c Desulfofervidia|o Desulfofervidales c Desulfobacteria c DSM-4660|o Desulfatiglandales c JADFXK01|g JADFXK01 c RBG-13-43-22|o RBG-13-43-22|f RBG-13-43-22 c Syntrophobacteria c Desulfarculia c Desulfobaccia|o Desulfobaccales c Zymogenia|o Zymogenales 100 100 100 98 100 100 92 100 100 100 100 100 100 99 100 100 96 100 0.1 10% 90% 55% 45% 62/1170 Methanoperedenaceae SRA out of 62 62/113 Desulfobacterota c. QYQDO1 SRA Methanoperedenaceae & Desulfobacterota c. QYQDO1 Co-existence not detected B Iron-rich subsurface Cadagno Sediment Hydrothermal vent SOURCE Co-existence: Methanoperedenaceae CYTOSOLIC HYDROGENASE NiFe Group 3c Present Absent A Meromictic lake water Hydrothermal sediment 1 2 3 1 2 3 c DTXE01 UWMA-0184 [GCA 012962595.1] c QYQD01 SpSt-1152 [GCA 011046395.1] Sed MAG c QYQD01 c QYQD01 DeMMO3_14 [GCA 020053325.1] c QYQD01 PowLak16_MAG11 [GCA 007280345.1] c JACQYL01|g JACQYL01 [GCA.016208165.1] c Syntrophia|o Syntrophales c Desulfomonilia c Thermodesulfobacteria|o Thermodesulfobacteriales c Dissulfuribacteria|o Dissulfuribacterales c Desulfobulbia|o Desulfobulbales c Desulfovibrionia|o Desulfovibrionales c Desulfofervidia|o Desulfofervidales c Desulfobacteria c DSM-4660|o Desulfatiglandales c JADFXK01|g JADFXK01 c RBG-13-43-22|o RBG-13-43-22|f RBG-13-43-22 c Syntrophobacteria c Desulfarculia c Desulfobaccia|o Desulfobaccales c Zymogenia|o Zymogenales 100 100 100 98 100 100 92 100 100 100 100 100 99 100 100 96 100 0.1 10% 90% 55% 45% 62/1170 Methanoperedenaceae SRA out of 62 62/113 Desulfobacterota c. QYQDO1 SRA Methanoperedenaceae & Desulfobacterota c. QYQDO1 Co-existence not detected B Iron-rich subsurface Cadagno Sediment Hydrothermal vent SOURCE Co-existence: Methanoperedenaceae CYTOSOLIC HYDROGENASE NiFe Group 3c Present Absent A Meromictic lake water Hydrothermal sediment 1 2 3 1 2 3 Figure 5. A Phylogenomic tree of the Desulfobacterota phylum based on Metagenome Assembled Genomes (MAG) with collapsed clades except for class QYQD01 and class DTXE01. The tree was generated via GTDB-Tk classification tools using multiple sequence alignments via concatenation of 120 bacterial phylogenetic markers. One MAG was included in addition to the genomes retrieved from the GTDB database: GCA_020053325.1 (Casar et al., 2021). Bootstrap values are shown for > 70% branching support. Branch length represents the average number of amino acid substitutions per site. B From top left to right, sequence read archive (SRA) hits for Methanoperedeneaceae family and Desulfobacterota class QYQD01. Colors indicate percentages of Sequencing Read Archive (SRA) entries where bothgroups were found in the same data set (pink) or not (brown). Bottom pie-chart displays SRA source from where both groups were found present.
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