218 Chapter 7 and biofilms, as described by Murali et al. (2023). This search revealed the presence of cohesion subunit Scc3/SA, dockerin-like domain proteins and extracellular Contractile Injection Systems (eCIS) across all “Ca. Methanoperedens” MAGs investigated, which either could help in the cellular adhesion or intercellular communication between organisms (Supplementary Table 9). 0 10 20 30 40 50 Sed MAG 1 Sed MAG 2 Sed MAG 3 Sed MAG 5 MnO2 MHC proteins others multiple - high multiple - medium multiple - low mn - medium mn - low iron - high iron - medium iron - low omcz nanowires 0 10 20 30 40 50 Sed MAG 1 Sed MAG 2 Sed MAG 3 Sed MAG 5 MnO2 MHC proteins others multiple - high multiple - medium multiple - low mn - medium mn - low iron - high iron - medium iron - low omcz nanowires 0 10 20 30 40 50 Sed MAG 1 Sed MAG 2 Sed MAG 3 Sed MAG 5 MnO2 MHC proteins others multiple - high multiple - medium multiple - low mn - medium mn - low iron - high iron - medium iron - low omcz nanowires 0 10 20 30 40 50 Sed MAG 1 Sed MAG 2 Sed MAG 3 Sed MAG 5 MnO2 MHC proteins others multiple - high multiple - medium multiple - low mn - medium mn - low iron - high iron - medium iron - low omcz nanowires Figure 4. Multi-heme c-type cytochrome (MHC) protein counts in the Lake Cadagno “Ca. Methanoperedens sp.” MAGs. Annotations are based on similarity (>70% to domain MHC) to reference “Ca. Methanoperedens” enrichment species that showed MHC expression under different electron accepting conditions - multiple (including electrode), manganese or iron oxides – and expression levels. “High” expression refers to the top 10% of the MHC transcripts, “medium” to the following 40%, and “low” to the remaining MHC transcript expression. OmcZ-like nanowire subunits are included as a separate category. The “Others” category includes MHC genes from this study that lack domain-homology-based expression links to reference “Ca. Methanoperedens” MAGs. Comparing the similarity of MHC proteins among the Lake Cadagno “Ca. Methanoperedens” spp. (Supplementary Figure 4) revealed that they shared between 9 and 16 homologous MHC proteins. For details see Supplementary Figure 4. Phylogenetic, biogeography and genomic analysis of the putative S-AOM partner Desulfobacterota class QYQD01 After the striking co-occurrence of the SRB Desulfobacterota class QYQD01 with “Ca. Methanoperedens” in Lake Cadagno sediments, we took a closer look at the Desulfobacterota class QYQD01 MAG. We observed that the class QYQD01 and the neighboring class DTXEO1 form a distinct clade within the Desulfobacterota
RkJQdWJsaXNoZXIy MTk4NDMw