Anna Brouwer

Prolonged cone b-IT in relation to retinal layer analysis identified, and the segmentation of these OCT images was manually checked and corrected when necessary. After manual correction, the thickness analysis was performed again and the thicknesses of the different retinal layers were compared between uveitis eyes with a prolonged cone b-IT, uveitis eyes with a normal cone b-IT, and eyes with no uveitis (of patients with a unilateral uveitis). Also, correlations between the implicit times of the cone b-wave of the different flash strengths (0.3, 1.0, 3.0 and 10.0 cds/m 2 ) in ms and thicknesses in microns were investigated. We considered p-values of < 0.05 as statistically significant. All tests were 2-tailed. Next, layer thickness were investigated in relation to clinical parameters that were associated with a prolonged cone b-IT including: fluorescein angiography (FA) scores (graded according to the Angiography Scoring for Uveitis Working Group (ASUWOG)), 14 the presence of cells in the anterior chamber (trace or more), or vitreous (1+ or more), as graded according the standardization of uveitis nomenclature (SUN) criteria, 15 and age, using Spearman’s rho coefficient. Figure 1: Example of the ten segmented retinal layers and the Early Treatment Diabetic Retinopathy Study (ETDRS) grid. On the right a schematic representation of the different cell types per layer. Abbreviations: RNFL = retinal nerve fiber layer, GCL = ganglion cell layer, IPL = inner plexiform layer, INL = inner nuclear layer, OPL = outer plexiform layer, ONL = outer nuclear layer, IS/OS junction = inner segment/outer segment junction, OSL = outer segment layer, OPR = outer segment photoreceptors/retinal pigment epithelium complex (OPR) and retinal pigment epithelium (RPE). RNFL GCL Axons of Ganglion Cells Nuclei of Ganglion Cells Synapses of Ganglion Cells and Bipolar Cells Nuclei of Bipolar Cells Synapses of Bipolar Cells and Photoreceptors ONL OPL ONL IS/OS junction OSL OPR RPE INL Nuclei of Photoreceptors Junction between Inner and Outer Segments of Photoreceptors Outer segments of Photoreceptors Retinal Pigment Epithelium Outer segments of Photoreceptors / Retinal Pigment Epithelium complex 1 2 4 8 6 9 5 3 7 1mm 3mm 6mm 97 4